Composite

Part:BBa_K4192110:Design

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)


cdg-gacA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
    Illegal BamHI site found at 259
    Illegal XhoI site found at 357
    Illegal XhoI site found at 801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 192
    Illegal NgoMIV site found at 1289
    Illegal AgeI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 210
    Illegal BsaI.rc site found at 777


Design Notes

Before using this part, please add appropriate promoters upstream, such as lac promoter,


Source

The cdg and gacA come from Pseudomonas fluorescens 2P24. The rbs sequence (BBa_K4192000) is synthetic.

References